Job Description

2025 Chromatography Method Development Intern Overview

Envision spending your summer working with energetic colleagues and inspirational leaders, all while gaining world-class experience in one of the most dynamic organizations in the pharmaceutical industry. This is a reality for AbbVie Interns.

The Drug Metabolite Screening Intern will support the Drug Metabolism and Disposition group in building and optimizing methods on high-end LC-MS systems to maximize the pipeline impact of our assays. The intern will modernize our current high-throughput metabolite ID LC-MS/MS workflows to improve separation of drug metabolites in liver microsomes, liver S9, and in vivo PK samples while reducing gradient length and increasing robustness across days of injection time. They will also evaluate control compounds and assay  parameters to determine whether they are adequate for current usage. The end result should be improved LC-MS/MS methods enabling metabolite soft-spot analysis across multiple therapeutic areas in the AbbVie discovery pipeline.

This position will be based in North Chicago, IL.

Key responsibilities include:

Evaluate current LC methods and protocols and identify key parameters to optimize, with a focus on column chemistry, solvents, and gradient length.

Develop robust LC methods with improved, consistent separation and test across a broad range of chemical space using commercial and internal compounds in relevant complex matrices.

If multiple methods are required, define appropriate conditions to use each method (chemical space, number of metabolites, ionization polarity, etc.)

Compile historic control data and propose QC cutoffs consistent with data utilization.

Present results to department and present at internal poster session.