1. Baseline Monitoring

* Working to existing protocol, take samples throughout the facility, prepare and incubate isolates.

* Train a small team in sample collecting, incubation, basic morphology, and gram staining

* Carry out morphology tests and identification and subsequent gram staining

* Carry out preparation of pure samples for external speciation

* Assist us with writing the final report, likely taking the lion's share of the work as we are not experienced in the appropriate contents required

2. Disinfectant Validation

* Prepare and incubate as required pure cultures from those we purchase, standard compendials.

* Prepare and incubate as required any specific cultures (as yet not speciated due to time constraints) that appear in our baseline monitoring and are not covered by the compendial isolates.

* Carry out the coupon testing against three disinfectants including all dilutions etc. A protocol exists

* If of course we have failures we will need to revisit parts of the exercise

* Write the final report

3. In operation/at rest monitoring as part of the cleaning validation and ongoing

* Check our SOP for active air sampling, use of machine and post-processing

* Incubate and isolate samples as with the baseline monitoring

4. Growth promotion testing of any batches of agar plates

* Preparation of pure cultures or use existing (from Biomerieux Bioballs) to test plates and any broths. Protocol exists